yes, but you will need to buy a new back cover, because the 160GB hard drives are a few mm thicker, so you probably want to buy the back cover for a 160GB classic. as i am aware, ipod classics should support bigger drives than the one they shipped with, as long as they can fit in the case and are 1.8" and use the same connector, when you install the drive, you will boot to the sad face, then use itunes to restore the ipod, but there is a chance that itunes will format the disk to 120GB, so be carefull