This will most likely not work, as the USB Subwoofer has to be supported by your operating system. As far as I remember Apple stopped support for those USB subwoofers a long time ago and in addition to that they were only supported on few computers (like the second generation iMacs). Even if it would work, you'd have two separate volumes – one for the subwoofer and one on the Pro Speakers amp. It's a pity. Long ago I had an iMac "snow" in combination with this USB iSub and it sounded terrfic. What you could do is to disassemble the sub and connect the speaker directly with the amp where you also hook up the Pro Speakers, but this requires a little bit more technical insight and I would not recommend to do it.
I'm a Mac aficionado from south Germany, born in '86. I love the Apple ecosystem from the early 90s up to the early 2000s. Currently I'm restoring old Macs in my small lab, to preserve this legacy.